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Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and <t>HepG2</t> cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).
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Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and <t>HepG2</t> cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).
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Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and HepG2 cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).

Journal: Frontiers in Oncology

Article Title: A novel auto-fluorescent porphyrin-lipid nanoparticle strategy for CTNNB1 gene silencing in hepatocellular carcinoma

doi: 10.3389/fonc.2026.1779803

Figure Lengend Snippet: Porphyrin-LNP synthesis and characterization. (A) Porphyrin-LNPs were synthesized via microfluidic rapid mixing. (B) . Cryo-TEM image of porphyrin-LNP (scale bar = 100 nm). (C) Size distribution of porphyrin-LNP-si-Scramble and (D) porphyrin-LNP-si-CTNNB1 measured by dynamic light scattering (DLS). (E) Confocal imaging of FAM-siRNA delivery by porphyrin-LNP. Hep3B and HepG2 cells were treated with porphyrin-LNP encapsulating FAM-siRNA (200 nM, ~2 μM porphyrin) for 6 h. Confocal images show FAM-siRNA (green) and porphyrin (magenta).

Article Snippet: The Hep3B (HB-8064) and HepG2 (HB-8065) human liver cancer cell lines were obtained from the American Type Culture Collection (ATCC) and tested negative for mycoplasma contamination.

Techniques: Synthesized, Imaging

Validation of CTNNB1 knockdown efficiency using porphyrin-LNP-siRNA. Downregulation of CTNNB1 expression after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (A) Hep3B and (B) HepG2 cells. **** p < 0.0001; one-way ANOVA test. Reduced colony formation after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (C) Hep3B and (D) HepG2 cells. * p < 0.05, *** p < 0.001, one-way ANOVA test.

Journal: Frontiers in Oncology

Article Title: A novel auto-fluorescent porphyrin-lipid nanoparticle strategy for CTNNB1 gene silencing in hepatocellular carcinoma

doi: 10.3389/fonc.2026.1779803

Figure Lengend Snippet: Validation of CTNNB1 knockdown efficiency using porphyrin-LNP-siRNA. Downregulation of CTNNB1 expression after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (A) Hep3B and (B) HepG2 cells. **** p < 0.0001; one-way ANOVA test. Reduced colony formation after transfection of porphyrin-LNP-siRNA targeting CTNNB1 in (C) Hep3B and (D) HepG2 cells. * p < 0.05, *** p < 0.001, one-way ANOVA test.

Article Snippet: The Hep3B (HB-8064) and HepG2 (HB-8065) human liver cancer cell lines were obtained from the American Type Culture Collection (ATCC) and tested negative for mycoplasma contamination.

Techniques: Biomarker Discovery, Knockdown, Expressing, Transfection